目的 采用HPLC指纹图谱、化学模式识别和多指标成分含量测定相结合的方法,对不同居群鸡血藤药材进行综合质量研究,为其质量评价提供参考。方法 收集了5个种源地共15个居群的鸡血藤药材样品,采用HPLC指纹图谱进行分析,结合相似度评价、聚类分析、主成分分析和正交偏最小二乘判别分析进行不同居群鸡血藤药材质量评价,并测定原儿茶酸、儿茶素、表儿茶素、染料木素和芒柄花素5种指标性成分的含量,同时按照《中国药典》的热浸法测定不同居群鸡血藤样品的醇溶性浸出物。结果 15个居群鸡血藤药材共标定了13个共有峰,经对照品比对指认了8个成分,指纹图谱相似度范围为0.692~0.984,具有较好的相似性;聚类分析将样品分为3类;主成分分析用3个主成分进行评价,综合得分显示,产自广东梅州、广西贺州和百色的鸡血藤质量较好;正交偏最小二乘判别分析筛选了不同产地鸡血藤药材的6个质量差异标志物,分别为芒柄花素、毛蕊异黄酮、染料木素、大豆苷元、色谱峰2和色谱峰3。含量测定结果表明不同产地鸡血藤药材中5种指标性成分含量存在明显差异;5种指标性成分的总含量与主成分综合得分存在明显相关性。结论 将HPLC指纹图谱、化学模式识别和多指标成分含量测定相结合的方法不仅可为鸡血藤药材质量控制的综合评价提供参考,也为其种质资源的品质评价提供了一定的科学依据。
Abstract
OBJECTIVE To study comprehensively the quality of Spatholobus suberectus Dunn from different populations based on the methods of HPLC fingerprint, chemical pattern recognition and quantitative analysis of multicomponents, to provide reference for its quality evaluation. METHODS The samples of Spatholobus suberectus Dunn from 15 populations in 5 provenances were collected and analyzed by HPLC fingerprints. The quality of Spatholobus suberectus Dunn from different populations was evaluated by similarity evaluation, cluster analysis, principal component analysis and orthogonal partial least squares discriminant analysis, and the contents of protocatechuic acid, catechin, epigallocatechin, genistein and fermononetin were determined. At the same time, the ethanol-soluble extract of Spatholobus suberectus Dunn samples from different populations were determined according to the hot soak method of Chinese Pharmacopoeia. RESULTS Thirteen common peaks were identified in 15 populations of Spatholobus suberectus Dunn, and 8 components were identified by reference substances. The similarity of fingerprint was 0.692-0.984, indicating good similarity. The samples were divided into 3 groups by cluster analysis; principal component analysis results extracted 3 principal components. According to the composite score, the quality of Spatholobus suberectus Dunn from Guangdong Meizhou, Guangxi Hezhou and Guangxi Baise were better than others. And 6 kinds of marker compounds that cause the quality difference of Spatholobus suberectus Dunn were screened out through the orthogonal partial least square discriminant analysis, which were fermononetin, calycosin, genistein, daidzein and peaks 2 and 3 respectively. The content determination results showed that there were significant differences in the contents of 5 index components in Spatholobus suberectus Dunn from different places, and there was a significant correlation between the total content of the 5 index components and the comprehensive score of the principal components. CONCLUSION The method of combining HPLC fingerprint, chemical pattern recognition and quantitative analysis of multicomponents can not only provide a reference for the comprehensive evaluation of the quality control of Spatholobus suberectus Dunn, but also provide a certain scientific basis for the quality evaluation of its germplasm resources.
关键词
鸡血藤 /
指纹图谱 /
化学模式识别 /
含量测定 /
质量评价
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Key words
Spatholobus suberectus Dunn /
fingerprint analysis /
chemical pattern recognition /
content determination /
quality evaluation
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中图分类号：
R284
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参考文献
[1] Ch.P (2020) Vol Ⅰ(中国药典 2020年版.一部)[S]. 2020:202.
[2] XIAO X J, LI M M, ZHAN R T, et al. Resource investigation and analysis of wild and cultivated Spatholobus suberectus[J]. J Chin Med Mater(中药材), 2022, 45(7):1582-1588.
[3] WANG C L, PAN C L, YAO L X, et al. Research progress on chemical constituents and pharmacological action of Spatholobus suberectus Dunn[J]. Res Pract Chin Med(现代中药研究与实践), 2021, 35(2):96-102.
[4] TAN X, DONG X Z, GUO D H, et al. Anti-radiation effect and mechanism studies of ethanol extracts from Spatholobus suberectus and its active component catechin[J]. China J Chin Mater Med(中国中药杂志), 2016, 41(9):1718-1724.
[5] WANG D, LIU P, CHEN R, et al. Effect of monomers extracted from Spatholobus suberectus Dunn on proliferation of hematopoietic progenitor cells in marrow-depressed mice[J]. Chin J Tissue Eng Res(中国组织工程研究与临床康复), 2008, 12(21):4163-4166.
[6] XU Z X, YANG Y, BIAN X, et al. Research progress on metabolic transformation and functional activity of genistein[J]. J Food Saf Qual(食品安全质量检测学报), 2022, 13(5):1359-1366.
[7] BAI Y, WANG H F, HUANG H P. Advance on pharmacological action of formononetin[J]. Drugs Clin(现代药物与临床), 2022, 37(2):425-432.
[8] ZHAI M, LIU J M, AN R, et al. Content determination of protocatechuic acid in Spatholobus suberectus Dunn[J]. Tradit Chin Drug Res Clin Pharmcol(中药新药与临床药理), 2009, 20(5):462-465.
[9] LU X L, PAN X J, DENG M M, et al. TLC identification and determination of catechin and epicatechin in Spatholobi Caulis extract[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2018, 24(18):88-92.
[10] YANG T C, LIN Z K, DING P, et al. Studies on systematical quality evaluation of Spatholobus suberectus[J]. Chin Pharm J(中国药学杂志), 2009, 44(23):1765-1768.
[11] CHEN H Y, YANG P, ZHANG M, et al. HPLC fingerprint of Spatholobus suberectus Dunn from different habitats and analysis of fermononetin content[J]. J Guangzhou Univ Tradit Chin Med(广州中医药大学学报), 2015, 32(5):923-928,936.
[12] XIONG J, LI H Y, LI G S, et al. Discussion on the application of chemometrics in chromatographic analysis of traditional Chinese medicine[J]. Chin J Pharm Anal(药物分析杂志), 2021, 41(10):1681-1689.
[13] Ch.P (2020) Vol Ⅰ(中国药典2020年版.一部)[S]. 2015:194.
[14] LIU J M, AN R, ZHA M, et al. Quality assessment of medicinal material of Caulis Spatholobi[J]. Tradit Chin Drug Res Clin Pharmcol(中药新药与临床药理)2012, 23(5):573-575.
[15] LIANG Y S, AN R, LIU J M, et al. Determination of genistein and fermononetin in Spatholobus suberectus Dunn from different places[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2013, 24(7):1655-1657.
[16] AN R. Analysis and evaluation of the quality of Caulis Spatholobi in GAP base[D]. Guangzhou:Guangzhou University of Chinese Medicine, 2011.
[17] DING P, YANG T C, LIN Z K, et al. Study on HPLC chromatographic fingerprint of Caulis spatholobi[J]. West China J Pharm Sci(华西药学杂志), 2010, 25(4):461-463.
[18] AN R, ZHAI M, SUN L X, et al. Study on the dynamic accumulation and change of secondary metabolites in the stems of cultivated Spatholobus suberectus Dunn[J]. J Guangzhou Univ Tradit Chin Med(广州中医药大学学报), 2015, 32(3):485-488.
[19] ZHAI M, LIU J M, AN R, et al. RAPD Analysis of Spatholobus suberectus Dunn with different germplasm[J]. Tradit Chin Drug Res Clin Pharmcol(中药新药与临床药理), 2010, 21(4):413-415.
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脚注
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基金
广东省省级乡村振兴战略(农业科技创新及推广体系建设)专项资助(2023KJ148);广东省普通高校“服务乡村振兴计划”重点领域专项资助(2019KZDZX2017);广东省教育厅 2021 年广东省本科高校教学质量与教学工程改革建设项目《中药学类中药材生产实践基地》资助(粤教高函[2021]29号)
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